CETRIMIDE AGAR

Technical Data #1229a / 98.04.30

Cetrimide Agar is a solid selective medium used for the isolation and identification of Pseudomonas aeruginosa. Cetrimide inhibits bacterial growth except Pseudomonas aeruginosa and enhances fluorescein and pyocyanin pigment production.

FORMULA
in grams per liter purified filtered water

Gelatin Peptone	20,0
Potassium Sulfate	10,0
Magnesium Chloride	1,4
Cetrimide	0,3
Agar	13,6
Glycerol	10 ml

pH 7,2 +/- 0,2 à 25º C

This approximate formula may be adjusted and/or enriched to obtain best results.

PRECAUTIONS
This medium is for IN VITRO diagnostic use only.

STORAGE
Store prepared media at 2-8º C protected from direct light and dehydrated powder, in a dry place, in tightly-sealed containers at 2-25º C.

SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.

DIRECTIONS
Suspend 45,3 g of dehydrated media in 1000 ml of purified filtered water. Heat with frequent agitation and boil for one minute. Add 10 ml of Glycerol (#8467). Sterilize at 121º C for 15 minutes. Cool to 45-50º C. Mix gently and dispense into sterile Petri dishes or sterile culture tubes.

PROCEDURE
Prior to inoculate, the prepared media should be brought to room temperature.

1. Directly, inoculate plates with specimens and streak for isolation. Tubes used for identification, should be inoculated with a 24 hours growth.
2. Incubate at 35º C for 24-48 hours.

QUALITY CONTROL
Results after 24 hrs at 35ºC

Organisms	ATCC	Growth	Pigmentation
Pseudomonas aeruginosa	27853	+	positive
Pseudomonas putida	12633	+	negative
Xanthomonas maltophilia	13637	-	negative
Escherichia coli	25922	- or partial	negative

LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.

REFERENCES

1. Brown, V.I., Lowbury, E.J.L. 1965 Use of an improved cetrimide agar medium and of culture methods for Pseudomonas aeruginosa J. Clin. Pathol. 18: 752
2. King, E.O., M.K. Ward, D.E. Raney. 1954 Two simple media for the demonstration of pyocyanin and fluorescein. J. Lab. Clin. Med. 44:301-307
3. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.: American society for Microbiology.
4. Mac Faddin, Jean F., Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria Vol.1 1985 Baltimore, MD. Williams & Wilkins.
5. Washington, J.A. Laboratory Procedures in Clinical Microbiology 1981. Springer-Verlag, New York.

CATALOGUE NUMBERS

Dehydrated media	500 g	QB-39-0806
Glycerol	100 ml	8467
Prepared media
Plates, 100x15 mm	10/pkg	1229
Mono-Plates, 100x15 mm	10/pkg	1229M
Tubes, 16x125 mm	10/pkg	2791

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