CONTACT: SABOURAUD DEXTROSE AGAR

Technical Data #1291a / 98.05.04

Contact Sabouraud Dextrose Agar is a solid medium used for the cultivation and enumeration of pathogenic and non pathogenic yeasts and molds. Carbohydrate is an enrichment and low pH inhibits bacterial growth.

FORMULA
in grams per liter purified filtered water

Casein Peptone	5,0
Meat Peptone	5,0
Dextrose	40,0
Agar	17,0

pH 5.6 +/- 0,2 à 25º C

This approximate formula may be adjusted and/or enriched to obtain best results.

PRECAUTIONS
This medium is for IN VITRO diagnostic use only.

STORAGE
Store prepared media at 2-8º C protected from direct light and dehydrated powder, in a dry place, in tightly-sealed containers at 2-25º C.

SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.

DIRECTIONS
Suspend 67 g of dehydrated media in 1000 ml of purified filtered water. Heat with frequent agitation and boil for one minute. Sterilize at 118º C for 15 minutes. Cool to 45-50º C. Mix gently and dispense into sterile Contact Petri dishes with a positive meniscus.

PROCEDURE
Prior to inoculate, the prepared media should be brought to room temperature.

1. Apply the plate's agar surface directly to the surface being tested with a gentle pressure. Roll the plate if a curved surface is tested.
2. Incubate plates at 35º C, for 7 days and at room temperature for up to 21 days.
3. Count the colonies.

QUALITY CONTROL
Results after 7 days at 25ºC

Organisms	ATCC	Growth
Candida albicans	60193	+
Trichophyton mentagrophytes	9533	+

LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.

REFERENCES

1. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.: American society for Microbiology.
2. Mac Faddin, Jean F., Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria Vol.1 1985 Baltimore, MD. Williams & Wilkins.
3. NCCLS 1990 Quality Assurance for Commercially Prepared Microbiological Culture Mediaicrobiology. Approved Standard Document M22-A Vol.10, No.14

CATALOGUE NUMBERS

Dehydrated media	500 g	QB-39-3806
Prepared media
Plates, 65x15 mm	10/pkg	1291

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