BRUCELLA 5% SHEEP BLOOD AGAR

Technical Data #1310a / 2000.12.19

Brucella Agar with 5% Sheep Blood is an enriched nonselective solid medium used for cultivation and isolation of nonfastidious and some fastidious microorganisms. Yeast extract is used as a source of vitamins source, dextrose provides carbon, sodium bisulfite is a reducing agent and sodium chloride maintains the osmotic equilibrium. The addition of sheep allows detection of hemolytic reactions.

 

 

FORMULA
in grams per liter purified filtered water

Casein Peptone

10,0

Meat Peptone

10,0

Yeast Extract

2,0

Dextrose

1,0

Sodium Chloride

5,0

Sodium Bisulfite

0,1

Agar

15,0

Sheep Blood

50 ml

pH 7,0 +/- 0,2 à 25º C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for IN VITRO diagnostic use only.


STORAGE
Store prepared media at 2-8° C protected from direct light and dehydrated powder, in a dry place, in tightly-sealed containers at 2-25° C.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.


DIRECTIONS
Suspend 43 g of dehydrated media in 950 ml of purified filtered water. Heat with frequent agitation and boil for one minute. Sterilize at 121° C for 15 minutes. Cool to 45-50° C and aseptically, add 50 ml of Sheep Blood (4245). Mix gently and dispense into sterile Petri dishes.


PROCEDURE
Prior to inoculate, the prepared media should be brought to room temperature.

  1. Directly, inoculate plates as soon as possible and streak for isolation.
  2. Incubate at 35° C for 24 to 72 hours in aerobic atmosphere supplemented or not supplemented with CO2
  3. Examine for growth and hemolytic activities.


QUALITY CONTROL
Results after 24 hrs at 35ºC w/CO2

Organisms

ATCC

Growth

Blackening

Streptococcus faecalis

29212

+

+

Streptococcus pyogenes

19615

- or partial

-

Escherichia coli

25922

- or partial

-


LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.


REFERENCES

  1. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.: American Society for Microbiology.
  2. Sutter, V.L., D.M. Citron, M.A.C. Edelstein, S.M. Finegold Wadsworth Anaerobic Bacteriology Manual 4th ed C.V. Mosby St. Louis
  3. Finegold and Baron. 1986 Bailey and Scottís Diagnostic Microbiology 7th, ed. The C.V. Mosby Company, St. Louis.


CATALOGUE NUMBERS

Dehydrated media 500 g QB-39-0606
     
Prepared media    
Plates, 100x15 mm 10/pkg 1310
Mono-Plates, 100x15 mm 10/pkg 1310M
     
Sheep Blood 50 ml 4245

 

 


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