BAIRD PARKER AGAR

Technical Data #1447a / 98.05.07

Baird Parker Agar is used for the detection of coagulase-positive Staphylococcus aureus from foods or other materials. A clear halo around staphylococci colonies confirms coagulase-positive, tellurite and lithium are the inhibitors while glycine and pyruvate enhance staphylococci growth.

FORMULA
in grams per liter purified filtered water

Casein Peptone

10,0

Beef Extract

5,0

Yeast Extract

1,0

Lithium Chloride

5,0

Glycine

12,0

Sodium Pyruvate

10,0

Agar

17,0

Supplement

 

Egg Yolks Tellurite

50 ml

pH 7,0 +/- 0,2 à 25º C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for IN VITRO diagnostic use only.


STORAGE
Store prepared media at 2-8º C protected from direct light and dehydrated powder, in a dry place in tightly-sealed containers at 2-25º C.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination (shrinking, cracking, evaporation or discoloration) Do not use dehydrated media if it is caked.


DIRECTIONS
Suspend 60 g in 950 ml of purified water. Heat with frequent agitation and boil for one minute. Sterilize at 121º C for 15 minutes. Cool to 45-50º C. Add 50 ml of Egg Yolk Tellurite Supplement (#8651). Mix thouroughly and dispense into Petri dishes.


PROCEDURE
Bring prepared medium at room temperature before inoculation.

  1. Directly, inoculate the specimen and streak for isolated colonies.
  2. Incubate at 35º C for 24-48 hours.


QUALITY CONTROL
Expected results after 24 hrs at 35ºC

Organisms

ATCC

Growth

Halo

Staphylococcus aureus

25923

+ black colonies

+

Staphylococcus aureus

6538

+ black colonies

+

Stapylococcus epidermidis

12228

+/- black colonies

-

Escherichia coli

25922

-

 

Proteus mirabilis

12453

+ brown colonies

-


LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.


REFERENCES

  1. Baird Parker, A.C., 1962 An improved diagnostic and selective medium for isolating coagulase-positive staphylococci. J. Appl. Bacteriol. 25:12.
  2. Lennette, E.H., E.H. Spaulding, J.P. Truant 1974 Manual of Clinical Microbiology. 2th ed. Washington D.C.: American Society for Microbiology.
  3. Mac Faddin, Jean F. 1985 Media for Isolation-Cultivation Identification-Maintenance of Medical Bacteria. Vol. I, Baltimore, MD.: Williams & Wilkins.


CATALOGUE NUMBERS

Dehydrated media 500 g QB-39-0106
     
Egg-Yolk-Tellurite Supplement 100 ml 8651
     
Prepared media    
Plates, 100x15 mm 10/pkg 1447
Mono-Plates, 100x15 mm 10/pkg 1447M

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