CONTACT PLATE MYCOBIOTIC GENTAMYCIN AGAR

Technical Data #1482a / 98.05.12

Mycobiotic Gentamycin Agar is a solid selective medium for isolation and enumeration of pathogenic yeast and molds from materials highly contaminated with other fungi and bacteria.

FORMULA
in grams per liter purified filtered water

Soy Peptone	10,0
Dextrose	10,0
Cycloheximide	0,4
Chloramphenicol	0,05
Gentamycin	0,005
Agar	15,5

pH 5,6 +/- 0,2 à 25º C

This approximate formula may be adjusted and/or enriched to obtain best results.

PRECAUTIONS
This medium is for IN VITRO diagnostic use only.

STORAGE
Store prepared media at 2-8º C protected from direct light and dehydrated powder, in a dry place, in tightly-sealed containers at 2-25º C.

SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.

DIRECTIONS
Suspend 36 g of dehydrated media in 1000 ml of purified filtered water. Heat with frequent agitation and boil for one minute. Sterilize at 121º C for 15 minutes. Cool to 45-50º C. Add 10 ml of sterile Gentamicin (8639). Mix gently and dispense into sterile Contact plates with a positive meniscus.

PROCEDURE
Prior to inoculate, the prepared media should be brought to room temperature.

1. Apply the plate's surface directly to the surface, being tested with a gentle pressure. Roll the plate if a curved surface is tested.
2. Incubate at 35º C for 7 days and at room temperature for 21 days.
3. Examine weekly and enumerate fungul growth.

QUALITY CONTROL
Results after 7 days at 35ºC

Organisms	ATCC	Growth
Trichophyton mentagrophytes	9533	+
Candida albicans	10231	+
Aspergillus niger	16404	- or partial
Escherichia coli	25922	- or partial

LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.

REFERENCES

1. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.: American society for Microbiology.
2. Mac Faddin, Jean F., Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria Vol.1 1985 Baltimore, MD. Williams & Wilkins.
3. NCCLS 1990 Quality Assurance for Commercially Prepared Microbiological Culture Media. Approved Standard Document M22-A Vol.10, No.14

CATALOGUE NUMBERS

Dehydrated media	500 g	QB-39-3020
Prepared media
Contact Plates 65x15mm	10/pkg	1482
Gentamicin Supplement	10x10 ml	8639

2331, Dandurand	Phone	Fax	Internet
Montreal (Quebec)	(514) 277-2558	(514) 277-4714	http://www.quelab.com
Canada H2G 3C5	1 (800) 579-0998	1 (800) 579-8177	info@quelab.com