CAMPYLOBACTER BLASER AGAR

Technical Data #1515a / 2000.09.25

Campylobacter Blaser Agar is a selective solid medium used for the cultivation and isolation of Campylobacter jejuni from fecal specimens. Addition of antibiotics, as suggested by Blaser, refrains normal stool flora overgrowth and enhances C. jejuni recovery.

FORMULA
in grams per liter purified filtered water

Casein Peptone

12,0

Meat Peptone

5,0

Yeast Extract

3,5

Beef Extract

3,0

Corn Starch

1,0

Sodium Chloride

5,0

Agar

13,5

Antibiotics

 
Cephalothin

0,015

Trimethoprim

0,005

Amphotericin B

0,002

Vancomycin

0,010

Polymyxin B

2500 units

Defibrinated Sheep Blood

75 ml

pH 7,4 +/- 0,2 à 25º C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for IN VITRO diagnostic use only.


STORAGE
Store prepared media at 2-8º C protected from direct light and dehydrated powder, in a dry place, in tightly-sealed containers at 2-25º C.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.


DIRECTIONS
Suspend 43 g in 925 ml of purified water. Heat with frequent agitation and boil for one minute. Sterilize at 121º C for 15 minutes. Cool to 45-50º C and aseptically add 75 ml of Defibrinated Sheep Blood (4895) and 2x5 ml of Blaser-Wang Supplement (8702). Dispense into sterile Petri dishes.


PROCEDURE
Prior to inoculate, the prepared media should be brought to room temperature.

  1. Directly, inoculate plate as soon as possible with specimen and streak for isolation. If inoculation will be delayed, an Enteric Pathogen Transport Medium as EPT-100 should be used.
  2. Incubate at 42º C under a reduced O2 atmosphere for 48 hours.


QUALITY CONTROL
Results after 48 hrs at 42º C

Organisms

ATCC

Growth

Campylobacter jejuni

33291

+

Escherichia coli

25922

- or partial

Proteus mirabilis

12453

- or partial

Enterococcus faecalis

29212

- or partial

Colonies of C. jejuni will appear on Campylobacter Blaser Agar as small, mucoid, flat or slightly raised, non-hemolytic translucent and gray. Some strains may vary from brown to tan.


LIMITATIONS OF METHOD
Since C. jejuni is thermophilic, plates should be incubated at, at least 42º C. This medium is only a part of the identification. Other tests may be required.


REFERENCES

  1. Blaser, M.J., I.V. Berkowitz, FM. LaForce, J. Cravens, L.B. Reller, and W-L. L. Wang. 1979 Campylobacter enteritis: clinical and epidimiologic features. Ann. Intern. Med. 91: 179-185.
  2. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.: American society for Microbiology.
  3. Mac Faddin, Jean F., Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria Vol.1 1985 Baltimore, MD. Williams & Wilkins.
  4. N.C.C.L.S. Quality Assurance for Commercially prepared Microbiological Culture Media. N.C.C.L.S. Publication M-22-A Approved Standard 1990 Vol. 10 No.14.


CATALOGUE NUMBERS

Dehydrated media 500 g QB-39-1005
     
Blaser-Wang Supplement 10x5 ml 8702
     
Defibrinated Sheep Blood 75 ml 4895
     
Prepared media    
Plates, 100x15 mm 10/pkg 1515
Mono-Plates, 100x15 mm 10/pkg 1515M
     
Enteric Pathogen Transport, Vial 100/cs EPT-100
     
Campylobacter Blaser Kit 6x1 L. QB-KT-0111

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