A-7 DIFFERENTIAL AGAR

Technical Data #1542a / 98.05.20

The A 7 Differential Agar is a selective solid medium used for the cultivation and isolation of Ureaplasma urealyticum and Mycoplasma sp. Fresh yeast extract, horse serum and enrichment enhance Ureaplasma and Mycoplasma growth. Penicillin G inhibits Gram positive bacteria.

FORMULA
in grams per liter purified filtered water

Casein Peptone	13,6
Soy Peptone	2,4
Potassium phosphate, dibasic	2,0
Sodium Chloride	4,0
L-Cysteine HCl	0,1
Dextrose	2,0
GHL, tripeptide	0,00002
Manganous Sulfate	0,15
Agar, Purified	10,5
Urea	1,0 g
Bio-X Enrichment	5,0 ml
Fresh Yeast Extract, pH 6,0	10,0
Penicillin G	1,0 MM units
Horse Serum, sterile	200,0 ml

pH 6,0 +/- 0,2 à 25º C

This approximate formula may be adjusted and/or enriched to obtain best results.

PRECAUTIONS
This medium is for IN VITRO diagnostic use only.

STORAGE
Store prepared media at 2-8º C protected from direct light.

SIGN OF DETERIORATION
Media should not be used if the expiry date has passed and if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration).

PROCEDURE
Prior to inoculate, the prepared medium should be brought to room temperature.

1. Urethral, vaginal, cervical swabs, urine sediment and other specimens are inoculated on Mycoplasma Ureaplasma Transport Medium (2281).
2. Transfer 0,1 ml of Mycoplasma-Ureaplasma Transport Medium into a U9 Broth tube (2361), 0,1 ml into Arginine Broth tube (2991) and 0,1 ml into A-7 Differential Medium (1542).
3. Incubate broths aerobically at 35º C.
4. Incubate plates at 35º C under reduced O2 conditions.
5. Examine broths and plates each day for up 7 days.

QUALITY CONTROL
Results after 3 days at 35º C

Organisms	ATCC	Growth
Ureaplasma urealyticum	27815	+
Ureaplasma urealyticum	27618	+
Mycoplasma hominis	23114	+

Microscopic examination at low power (100x) shows brown colonies as Ureaplasma and typical "fried egg" colonies with a brown nucleus as Mycoplasma.

LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.

REFERENCES

1. Balows, A., W.J. Hausler. 1981 Diagnostic Procedures for Bacterial, Mycotic and Parasitic Infections 6th ed.
2. Kenny, G.E., 1985 Manual of Clinical Microbiology, 4th ed. Chap. 36, pp. 407-411, American Society for Microbiology.
3. Lefebvre, J., Public Health Laboratory, D.S.C. St-Luc Hospital, Ste-Anne-de-Bellevue, Personnal Communications.
4. Mac Faddin, Jean F., Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria Vol.1 1985 Baltimore, MD. Williams & Wilkins.
5. Shepard, M.C. and C.D. Lanceford. 1976 Differential agar medium (A-7) for identification of Ureaplasma urealyticum (human T-strain) in primary cultures of clinical material. J. Clin. Microbiol. 3:613-625.

CATALOGUE NUMBERS

Prepared media
Plates 60x15 mm	10/pkg	1542
Mycoplasma Ureaplasma Transport
Tubes 12x75 mm	10/pkg	2281
Ureaplasma U9 Broth
Tubes 12x75 mm	10/pkg	2361
Mycoplasma Arginine Broth
Tubes 12x75 mm	10/pkg	2991

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