B.H.I.A. (BRAIN HEART INFUSION AGAR) W/ 5% SHEEP BLOOD

Technical Data #1627a / 98.06.10

B.H.I.A. w/ 5% sheep blood is highly nutritious general purpose medium for cultivation of large variety of non-fastidious microorganisms including yeasts and molds.

FORMULA
in grams per liter purified filtered water

Brain Heart Infusion	10,0
Casein Peptone	8,5
Meat Peptone	8,5
Sodium Chloride	5,0
Dextrose	2,5
Disodium Phosphate	2,5
Agar	15,0
Defibrinated Sheep Blood	50 ml

pH 7.4 +/- 0,2 à 25º C

This approximate formula may be adjusted and/or enriched to obtain best results.

PRECAUTIONS
This medium is for IN VITRO diagnostic use only.

STORAGE
Store prepared media at 2-8º C protected from direct light and dehydrated powder, in a dry place, in tightly-sealed containers at 2-25º C.

SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.

DIRECTIONS
Suspend 52 g of dehydrated media in 950 ml of purified filtered water. Heat with frequent agitation and boil for one minute. Sterilize at 121º C for 15 minutes. Cool to 45-50º C and aseptically, add 50 ml of Defibrinated Sheep Blood (4245). Mix gently and dispense into sterile Petri dishes or sterile culture tubes.

PROCEDURE
Prior to inoculate, the prepared media should be brought to room temperature.

1. Directly, inoculate plates as soon as possible with specimen and streak for isolation.
2. Incubate at 25 or 35º C as required for 2-7 days.
3. Examine for growth.

QUALITY CONTROL
Results after 4 days at 25ºC

Organisms	ATCC	Growth
Streptococcus pyogenes	19615	+
Candida albicans	10213	+
Candida albicans	60193	+
Trichophyton mentagrophytes	9533	+

LIMITATIONS OF METHOD
Subculture growth for identification. This medium is only a part of the identification. Other tests may be required.

REFERENCES

1. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.: American Society for Microbiology.
2. Finegold, S.M., E.J. Baron 1986 Bailey and Scott's Diagnostic Microbiology 7th ed. C.V. Mosby, St. Louis
3. Mac Faddin, Jean. F., 1985 Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria. Vol. 1. Baltimore, MD.: Williams & Wilkins.

CATALOGUE NUMBERS

Dehydrated media	500 g	QB-39-0206
Prepared media
Plates, 100x15 mm	10/pkg	1627
Mono-Plates, 100x15 mm	10/pkg	1627M
Tubes, 16x125 mm	10/pkg	2441
Defibrinated Sheep Blood	50 ml	4245

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