BILE ESCULIN AGAR

Technical Data #1638a / 98.06.10

Bile Esculin Agar is a selective culture medium used for the isolation of group D streptococci. Esculin hydrolysed by group D enterococci give a dark brown colour. Oxgall inhibits Gram-positive bacteria other than enterococci.

FORMULA
in grams per liter purified filtered water

Gelatin Peptone	5,0
Beef Extract	3,0
Oxgall	40,0
Esculin	1,0
Ferric Citrate	0,5
Agar	15,0
Horse Serum	50 ml

pH 6,6 +/- 0,2 à 25º C

This approximate formula may be adjusted and/or enriched to obtain best results.

PRECAUTIONS
This medium is for IN VITRO diagnostic use only.

STORAGE
Store prepared media at 2-8º C protected from direct light and dehydrated powder, in a dry place in tightly-sealed containers at 2-25º C.

SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. prepared media should not be used if there are signs of contamination (shrinking, cracking, evaporation or discoloration) Do not use dehydrated media if it is caked.

DIRECTIONS
Suspend 64 g in 950 ml of purified water. Heat with frequent agitation and boil for one minute. Sterilize at 121º C for 15 minutes. Cool to 45-50º C and add 50 ml of horse serum (4173). Mix thouroughly and dispense into Petri dishes or into sterile culture tubes.

PROCEDURE
Bring prepared medium at room temperature before inoculation.

1. Inoculate with 2-3 similar colonies.
2. Incubate at 35º C for 24 hours.

QUALITY CONTROL
Expected results after 24 hrs at 35ºC

Organisms	ATCC	Growth	Blackening
Streptococcus faecalis	29212	+	+
Streptococcus pyogenes	19615	- or partial	-

LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.

REFERENCES

1. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.: American society for Microbiology.
2. Mac Faddin, Jean F. 1985 Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria. Vol. I, Baltimore, MD.: Williams & Wilkins.
3. N.C.C.L.S. 1990 Quality Assurance for Commercially Prepared Microbiological Culture Media Approved Standard. Vol. 10, No. 14, NCCLS Document M22-A

CATALOGUE NUMBERS

Dehydrated media	500 g	QB-39-1115
Prepared media
Plates, 100x15 mm	10/pkg	1638
Mono-Plates, 100x15 mm	10/pkg	1638M
Tubes 16x125 mm, 5 ml	10/pkg	2540
	100/box	2540-H
Horse Serum	50 ml	4173

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