FASTIDIOUS ANAEROBE AGAR (F.A.A.)

Technical Data #1780a / 98.06.12

FAA Anaerobe Blood Agar is an enriched nonselective solid medium used for cultivation and isolation of obligate and facultative anaerobes especially present in low numbers. Hemin and Vitamin K1 enrichments enhance the growth of Bacteroides sp. The low level of glucose prevents the production of high levels of acids and alcohols which would inhibit colonial development.

FORMULA
in grams per liter purified filtered water

Casein Peptone	10,0
Meat Peptone	10,0
Yeast Extract	3,0
Dextrose	1,0
Starch	1,0
Sodium Chloride	5,0
Sodium Pyruvate	1,0
Sodium Bicarbonate	0,4
Cysteine HCl	0,5
L-Arginine	1,0
Trizma Base	0,25
Agar	14,0
Enrichments
Hemin	0,01
Vitamine K1	0,001
Sodium Succinate	0,5
Horse Blood	50 ml

pH 7,2 +/- 0,2 à 25º C

This approximate formula may be adjusted and/or enriched to obtain best results.

PRECAUTIONS
This medium is for IN VITRO diagnostic use only.

STORAGE
Store prepared media at 2-8º C protected from direct light and dehydrated powder, in a dry place, in tightly-sealed containers at 2-25º C.

SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.

DIRECTIONS
Suspend 47 g of dehydrated media in 950 ml of purified filtered water. Heat with frequent agitation and boil for one minute. Sterilize at 121º C for 15 minutes. Cool to 45-50º C and aseptically, add 50 ml of Horse Blood (4526), 2x5 ml of Vitamin K-Hemin-Succinate (#8730). Mix gently and dispense into sterile Petri dishes.

PROCEDURE
Prior to inoculate, the prepared media should be brought to room temperature.

1. Suitable anaerobes recovery is depending of collection and transportation of specimens. Specimens should be transferred into Anaerobes Pre-Reduced Transport Medium (2017) immediately after collection and brought to the laboratory quickly.
2. Directly, inoculate plate as soon as possible with a large amount of inoculum, streak for isolation.
3. Incubate at 35º C under anaerobic conditions for 2-4 days.
4. Examine for growth.

QUALITY CONTROL
Results after 48 hrs at 35ºC anaerobic atmosphere

Organisms	ATCC	Growth
Bacteroides fragilis	25285	+
Clostridium perfringens	13124	+ hemolysis
Fusobacterium nucleatum	25586	+
Bacteroides levii	29147	+
Peptostreptococcus anaerobius	27337	+

LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.

REFERENCES

1. Brazier , J.S. 1986 A note on ultra-violet red fluorescence of anaerobic bacteria in vitro. J.Appl. Bact. 60:121-126.
2. Brazier, J.S. 1986 Yellow fluorescence of fusobacteria. Letters in Appl. Microbiol. 2:125-126.
3. Eley, A., T. Clarry, K.W. Bennett 1989 Selective and differential medium for isolation of Bacteroides ureolyticus from clinical specimens. Eur.J. Clin. Microbiol. Infect. Dis Vol. 8:83-85
4. Heginbothom, M., T.C. Fitzgerald, W.G. Wade. 1990 Comparaison of solid media for the culture of anaerobes. J. Clin. Path. 43:253-256.
5. Wade, W., M. Griffith. 1987 Comparaison of media for cultivation of subgingival bacteria J. Dent. Res. 66: no.4, abstract 334.

CATALOGUE NUMBERS

Dehydrated media	500 g	QB-39-1718
Prepared media
Plates, 100x15 mm	10/pkg	1780
Mono-Plates, 100x15 mm	10/pkg	1780M
Vitamin K-Hemin-Succinate Enrichment, 5 ml	10/pkg	8730
Horse Blood	50 ml	4526

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