BILE ESCULIN AZIDE VANCOMYCIN AGAR

Technical Data #1880a / 2000.11.01

Bile Esculin Azide Vancomycin Agar is a selective screening
medium used for the isolation of esculin hydrolyzing Enterococcus faecalis Vancomycin Resistant.
Sodium azide inhibits gram-negative organisms while bile salts inhibit gram-positive strains other than enterococci and esculin hydrolysis gives black colonies.

FORMULA
in grams per liter purified filtered water

Casein Peptone	16,0
Meat Peptone	9,5
Oxbile	10,0
Esculin	1,0
Sodium Chloride	5,0
Sodium Citrate	1,0
Ferric Ammonium Citrate	0,5
Sodium Azide	0,25
Agar	14,0
Antibiotics
Vancomycin	0,006

pH 7,1 +/- 0,2 à 25° C

This approximate formula may be adjusted and/or enriched to obtain best results.

PRECAUTIONS
This medium is for IN VITRO diagnostic use only.

STORAGE
Store prepared media at 2-8° C protected from direct light and dehydrated powder, in a dry place in tightly-sealed containers at 2-25° C.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.

DIRECTIONS
Suspend 57 g in 1000 ml of purified water. Heat with frequent agitation and boil for one minute. Sterilize at 121° C for 15 minutes. Cool to 45-50° C. Add 2x5 ml of # 8736 VRE Vancomycin Supplement. Mix thouroughly and dispense into Petri dishes.


PROCEDURE
Bring prepared medium at room temperature before inoculation

1. Directly, inoculate the specimen and streak for isolated colonies.
2. Incubate at 35° C for 24-48 hours.

QUALITY CONTROL
Expected results after 24 hrs at 35ºC

Organisms	ATCC	Growth	Esculin Hydrolysis
Enterococcus faecalis	29212	-	-
Enterococcus faecalis	51299	+	black colonies
Enterococcus gallinarum	clinical	+	black colonies

LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.


REFERENCES

1. Ballows, A., Hausler, W.J.Jr., K.L Herrmann, H.D. Isenberg, Shadomy, H.J. 1991 Manual of Clinical Microbiology. 5th ed. Washington D.C.: American Society for Microbiology.
2. Sahm, D.F., S. Boonlayangoor, P.C. Iwen, J.L. Baade, G.L. Woods 1991 Factors influencing determination of High-Level Aminoglycoside resistance in Enterococcus faecalis. J. Clin. Microbiol. 29:1934-1939
3. Swenson, J.M., N.C. Clark, M.J. Ferraro, D.F. Sahm, G. Doern, M.A. Pfaller, L.B. Reller, M.P. Weinstein, R.J. Zabransky, F.C. Tenover 1994 Development of a Standardized Screening Method for Detection of Vancomycin-Resistant Enterococci J. Clin. Microbiol. 32:1700-1704
4. Tenover, F.C., J. Tokars, J. Swenson, S. Paul, K. Spitalny, W. Jarvis 1993 Ability of clinical laboratories to detect antimicrobial agent-resistant enterococci J. Clin. Microbiol. 31:1695-1699.

CATALOGUE NUMBERS

Dehydrated media	500 grams	QB-39-0118
VRE Vancomycin Supplement	10 vials/pkg	8736
Prepared media
Pétri 100x15 mm	10/pkg	1880
Mono-plates, 100x15 mm	10/pkg	1880M

2331, Dandurand	Phone	Fax	Internet
Montreal (Quebec)	(514) 277-2558	(514) 277-4714	http://www.quelab.com
Canada H2G 3C5	1 (800) 579-0998	1 (800) 579-8177	info@quelab.com