B.C.S.A. AGAR (BURKHOLDERIA CEPACIA SELECTIVE AGAR)

Technical Data #1995a / 2005.08.18

BCSA Agar is a solid and selective medium used for the isolation of Burkholderia cepacia from mixed microbial flora, from sputum specimens of cystic fibrosis patients. Crystal violet, gentamycin, polymyxin B and vancomycin are the inhibitors; phenol red is the pH indicator.

FORMULA
in grams per liter purified filtered water

Casein Peptone	10,0
Yeast Extract	1,5
Sodium Chloride	5,0
Lactose	10,0
Sucrose	10,0
Crystal Violet	0,002
Phenol Red	0,08
Agar	14,0
Antibiotics	0,01
Gentamycin
Polymyxin B	600 000 u
Vancomycin	0,0025

pH 7,0 +/- 0,2 à 25º C

This approximate formula may be adjusted and/or enriched to obtain best results.

PRECAUTIONS
This medium is for laboratory use only.

STORAGE
Store prepared media at 2-8° C protected from direct light.

SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration).

PROCEDURE
Prior to inoculate, the prepared media should be brought to room temperature.

1. Directly, inoculate plates as soon as possible and streak for isolation.
2. Incubate at 35° C for 48-72 hours.
3. Examine daily for growth.

QUALITY CONTROL
Results after 48 hrs at 35ºC

Organisms	ATCC	Growth
Burkholderia cepacia	25416	+
Pseudomonas aeruginosa	27853	-
Staphylococcus aureus	25923	-
Escherichia coli	25922	-

LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.

REFERENCES

1. Speert, D.P., D.A. Henry, M.E. Campbell and J.J. LiPuma 1997 Identification of Burkholderia cepacia isolates from patients with cystic fibrosis and use of a simple new selective medium J. Clin. Microbiol. 35:614-619.
2. Gilligan, P.H., P.A. Gage, L.M. Bradshaw, D.V. Schidlow, B.T. DeCicco 1985 Isolation medium for the recovery of Pseudomonas cepacia from respiratory secretions of patients with cystic fibrosis. J. Clin. Microbiol. 22:5-8.
3. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.: American society for Microbiology.
4. Speert, D.P., D.A. Henry, M.E. Campbell and J.J. LiPuma 1997 Identification of Burkholderia cepacia isolates from patients with cystic fibrosis and use of a simple new selective medium J. Clin. Microbiol. 35:614-619.
5. Tablan, O.C., L.A. Carson, L.B. Cusick, L.A. Bland, W.J. Martone, W.R. Jarvis 1987 Laboratory proficiency test results on use of selective media for isolating Pseudomonas cepacia from simulated sputum specimens of patients with cystic fibrosis. J.Clin. Microbiol. 25:485-487.
6. Welch, D.F., M.J. Muszynski, C.H. Pai, M.J. Marcon, M.M Hribar, P.H. Gilligan, J.M. Matsen, P.A. Ahlin, B.C. Hillman S.A. Chartrand 1987 Selective and differential medium for recovery of Pseudomonas cepacia from the respiratory tracts of patients with cystic fibrosis. J. Clin. Microbiol. 25:1730-1734

CATALOGUE NUMBERS

Prepared media
Plates, 100x15 mm	10/pkg	1995
Mono-Plates, 100x15 mm	10/pkg	1995M

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