B.C.P.(BROM CRESOL PURPLE) BROTH WITH CARBOHYDRATES

Technical Data #2063a / 2004.11.22

BCP Broths are liquid medium, supplemented with carbohydrates, used to determine the carbohydrate fermentation patterns of enteric gram-negative bacilli. Carbohydrate fermentation turns yellow pH indicator and Durham tubes are provided to entrap gas.

FORMULA
in grams per liter purified filtered water

Gelatin Peptone

5,0

Meat Peptone

5,0

Sodium Chloride

5,0

Brom Cresol Purple

0,015

Specific Carbohydrate

10,0

pH 6,8 +/- 0,2 à 25º C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for laboratory use only.


STORAGE
Store prepared media at 2-8º C protected from direct light and dehydrated powder, in a dry place, in tightly-sealed containers at 2-25º C.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (evaporation or discoloration). Do not use dehydrated media if it is caked.


DIRECTIONS
Suspend 15 g of dehydrated media in 900 ml of purified filtered water. Heat gently to dissolve. Add 100 ml of 10% Dextrose, Trehalose, Adonitol etc. solution. For control broth, add 100 ml of purified filtered water. Sterilize at 118º C for 15 minutes. AVOID OVERHEATING. Cool to 45-50º C. Mix gently and dispense into sterile culture tubes containing Durham tube.


PROCEDURE
Prior to inoculate, the prepared media should be brought to room temperature.

  1. Inoculate tubes with a 1-2 similar colonies from an overnight growth.
  2. Incubate at 35º C for up to 3-4 days.
  3. Examine daily for acidification.


QUALITY CONTROL
Results after 48 hrs at 35ºC

Organisms

ATCC

Reaction

     
CONTROL

 

 

Escherichia coli

25922

-

Edwardsiella tarda

15947

-

     
TREHALOSE

 

 

Escherichia coli

25922

+

Edwarsiella tarda

15947

-


LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.


REFERENCES

  1. Finegold, S.M., E.J. Baron 1986 Bailey and Scott's Diagnostic Microbiology 7th ed. C.V. Mosby, St. Louis
  2. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.: American society for Microbiology.
  3. Mac Faddin, Jean F., Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria Vol.1 1985 Baltimore, MD. Williams & Wilkins.


CATALOGUE NUMBERS

Dehydrated media 500 g QB-39-3710
Dextrose, granulated 500 g QB-39-1210
Trehalose 10 g QB-60-5251
     
Prepared media    
Tubes, 16x125 mm, 8 ml w/ Durham tube    
Control 10/pkg 2063
Adonitol 10/pkg 2268
Arabinose 10/pkg 2624
Cellobiose 10/pkg 2186
Dextrose 10/pkg 2254
Dextrin 10/pkg 2806
Dulcitol 10/pkg 2060
Fructose 10/pkg 2205
Galactose 10/pkg 2687
Inositol 10/pkg 2639
Inulin 10/pkg 2850
Lactose 10/pkg 2945
Maltose 10/pkg 2195
Melibiose 10/pkg 2511
Mannitol 10/pkg 2134
Raffinose 10/pkg 2729
Rhamnose 10/pkg 2307
Salicin 10/pkg 2180
Sorbitol 10/pkg 2915
Sorbose 10/pkg 2598
Sucrose 10/pkg 2078
Trehalose 10/pkg 2890
Xylose 10/pkg 2568

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