Technical Data #2105a / 2013.06.23
Malonate Broth is a medium used for the differentiation of microorganisms on the basis of their ability to utilize malonate with resultant alkalinity and blue color in the medium.
in grams per liter purified filtered water
|Potassium Phosphate, dibasic||
|Potassium Phosphate, monobasic||
pH 6,7 +/- 0,2 à 25º C
This approximate formula may be adjusted and/or enriched to obtain best results.
This medium is for IN VITRO diagnostic use only.
Store prepared media at 2-8º C protected from direct light and dehydrated powder, in a dry place in tightly-sealed containers at 2-25º C.
SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination (evaporation or discoloration). Do not use dehydrated media if it is caked.
Suspend 9,3 g in 1000 ml of purified filtered water. Heat with frequent agitation and boil for one minute. Sterilize at 121º C for 15 minutes. Cool to 45-50º C. Mix thouroughly and dispense into sterile culture tubes.
Bring prepared medium at room temperature before inoculation.
Expected results after 24 hrs at 35ºC
LIMITATIONS OF METHOD
Any trace of blue color means positive reaction. A final negative result may be reported only after 48 hours of incubation. This is only a part of the identification. Others tests may be required.
|Dehydrated media||500 g||QB-39-2805|
|Tubes, 16X125 mm||10/pkg||2105|
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