ESCULINE BROTH

Technical Data #2156a / 98.06.23

Esculin Broth is a liquid selective medium used for the identification of Streptococcus group D or some anaerobes by their esculin hydrolysis ability. Broth blackening confirms a positive reaction.

FORMULA
in grams per liter purified filtered water

Meat Peptone	10,0
Ferric Citrate	1,0
Esculin	1,0

pH 7,0 +/- 0,2 à 25º C

This approximate formula may be adjusted and/or enriched to obtain best results.

PRECAUTIONS
This medium is for IN VITRO diagnostic use only.

STORAGE
Store prepared media at 2-8º C protected from direct light and dehydrated powder, in a dry place in tightly-sealed containers at 2-25º C.

SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. prepared media should not be used if there are signs of contamination (evaporation or discoloration). Do not use dehydrated media if it is caked.

DIRECTIONS
Suspend 12 g in 1000 ml of purified water. Heat with frequent agitation and boil for one minute. Mix thouroughly and dispense into culture tubes. Sterilize at 121º C for 15 minutes.

PROCEDURE
Bring prepared medium at room temperature before inoculation. If anaerobes should be inoculated, boil broth for 15 minutes with loosened caps and cool at room temperature, before inoculating.

1. Inoculate broth with 3-4 similar colonies.
2. Incubate at 35º C for 24-48 heures. Anaerobes should be incubated with tighten caps.
3. Examine for blackening of medium.

QUALITY CONTROL
Expected results after 24-48 hrs at 35ºC

Organisms	ATCC	Blackening
Pseudomonas aeruginosa	27853	-
Pseudomonas cepaciae	25416	+
Escherichia coli	25922	-
Enterococcus faecalis	29212	+
Bacteroides fragilis	25285	+

LIMITATIONS OF METHOD
This is only a part of the identification. Others tests may be required.

REFERENCES

1. Dowell, V.R. Jr., T.M. Hawkins 1977 Laboratory methods in anaerobic bacteriology. Department of Health, Education and Welfare Center for Disease Control, Atlanta.
2. Dowell, V.R. Jr., G.L. Lombard, F.S. Thompson, A.Y. Armfield 1977 Media for isolation, characterization and identification of obligately anaerobic bacteria. U.S. Department of Health, Education and Welfare Center for Disease Control, Atlanta
3. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.: American society for Microbiology.
4. Mac Faddin, Jean F., Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria Vol.1 1985 Baltimore, MD. Williams & Wilkins.

CATALOGUE NUMBERS

Dehydrated media	500 g	QB-39-2503
Prepared media
Tubes, 16X125 mm	10/pkg	2156

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