CAMPY-THIO MEDIUM (CAMPYLOBACTER THIOGLYCOLLATE)

Technical Data #2340a / 98.06.25

Campy-Thio Medium is a selective liquid medium used for recovering Campylobacter jejuni from fecal specimens when there is a delay in plating specimens. Addition of antibiotics, as suggested by Blaser, refrains normal stool flora overgrowth and enhances C. jejuni recovery.

FORMULA
in grams per liter purified filtered water

Casein Peptone

17,0

Soy Peptone

3,0

Dextrose

6,0

Sodium Chloride

2,5

Sodium Thioglycollate

0,5

L-Cystine

0,25

Sodium Sulfite

0,1

Agar

1,6

Cephalothin

0,015

Trimethoprim

0,005

Amphotericin B

0,002

Vancomycin

0,010

Polymyxin B

2500 units

pH 7.0 +/- 0,2 à 25º C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for IN VITRO diagnostic use only.


STORAGE
Store prepared media at 2-8º C protected from direct light.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed or if there are signs of contamination.


PROCEDURE
Prior to inoculate, the prepared media should be brought to room temperature.

  1. Swab: Place the swab 2 cm into medium and twirl the swab. Then, lower it to the bottom of the tube. Diarrheal Stools: Place 3-5 drops into the Campy-Thio Medium. Solids Stools: Place 3-5 drops of a saline suspension into the Campy-Thio Medium.
  2. Refrigerate overnight.
  3. Subculture by placing the tip of a sterile pipet about 2 cm below the surface of the broth and placing 3 drops on a Campylobacter Blaser Agar. Streak the plate to obtain isolated colonies.
  4. Incubate at 42º C under a reduced O2 atmosphere for 48 hours.


QUALITY CONTROL
Results after 48 hrs at 42ºC

Organisms

ATCC

Growth

Campylobacter jejuni

33291

+

Escherichia coli

25922

- or partial

Proteus mirabilis

12453

- or partial

Streptococcus faecalis

29212

- or partial

Colonies of C. jejuni will appear on Campylobacter Blaser Agar as small, mucoid, flat or slightly raised, non-hemolytic translucent and gray. Some strains may vary from brown to tan.


LIMITATIONS OF METHOD
Since C. jejuni is thermophilic, plates should be incubated at, at least 42º C. This medium is only a part of the identification. Other tests may be required.


REFERENCES

  1. Blaser, M.J., I.V. Berkowitz, FM. LaForce, J. Cravens, L.B. Reller, and W-L. L. Wang. 1979 Campylobacter enteritis: clinical and epidimiologic features. Ann. Intern. Med. 91: 179-185.
  2. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.: American Society for Microbiology.
  3. Luechtefeld, N.W., W-L.L. Wang, and M.J. Blaser and L.B. Reller. 1981 Evaluation of transport and storage techniques for isolation of Campylobacter fetus subsp. jejuni from turkey fecal specimens. J. Clin. Microbiol. 13:438-443.
  4. Mac Faddin, Jean F., Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria Vol.1 1985 Baltimore, MD. Williams & Wilkins.


CATALOGUE NUMBERS

Campy-Thio Medium 10 tubes/pkg 10 ml/tube 2340
  5 ml/tube 2381
     
Campylobacter Blaser    
Plates, 100x15 mm 10/pkg 1515
Mono-Plates, 100x15mm 10/pkg 1515M
     
Enteric Pathogen Transport, Vial 100/cs EPT-100

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