BRAIN HEART INFUSION BROTH W/ SODIUM CHLORIDE 6,5%

Technical Data #2368a / 98.06.25

BHI Infusion Broth with 6,5% Sodium Chloride is a liquid medium used to differentiate enterococci from other streptococci by addition of 65 grams per liter of sodium chloride. Sodium chloride reacts as selective agents because mostly of Enterococci sp. may grow in this selective broth. Bromcresol purple is the indicator.

FORMULA
in grams per liter purified filtered water

Beef Heart Infusion	12,0
Casein Peptone	3,5
Meat Peptone	3,5
Dextrose	2,5
Sodium Phosphate, dibasic	1,5
Sodium Chloride	68,0
Bromcresol Purple	0,008

pH 7,0 +/- 0,2 à 25º C

This approximate formula may be adjusted and/or enriched to obtain best results.

PRECAUTIONS
This medium is for IN VITRO diagnostic use only.

STORAGE
Store prepared media at 2-8º C protected from direct light and dehydrated powder, in a dry place in tightly-sealed containers at 2-25º C.

SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination (evaporation or discoloration). Do not use dehydrated media if it is caked.

DIRECTIONS
Suspend 91 g in 1000 ml of purified filtered water. Heat with frequent agitation to dissolve. Mix thouroughly and dispense into culture tubes. Sterilize at 121º C for 15 minutes.

PROCEDURE
Bring prepared medium at room temperature before inoculation.

1. Inoculate with 3-5 similar colonies from an overnight growth.
2. Incubate at 35º C for 48-72 hours.
3. Examine daily for growth.

QUALITY CONTROL
Expected results after 24 hrs at 35ºC

Organisms	ATCC	Growth	Color
Enterococcus faecalis	29212	+	yellow
Streptococcus pyogenes	19615	-	blue
Streptococcus bovis	9809	-	blue

LIMITATIONS OF METHOD
Negative result may be confirmed only after 3 days of incubation. This is only a part of the identification. Others tests may be required.

REFERENCES

1. Facklam, R.R., 1973 Comparaison of several laboratory media for presumptive identification of enterococci and group D streptococci. Appl. Microbiol. 26:138.
2. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.: American society for Microbiology.
3. Mac Faddin, Jean F. 1985 Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria. Vol. I, Baltimore, MD.: Williams & Wilkins.

CATALOGUE NUMBERS

Dehydrated media	500 g	QB-39-0303
Prepared media
Tubes 16x125 mm, 5 ml	10/pkg	2368
Tubes 16x125 mm, 5 ml	100/pkg	2368-H

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