B.H.I. ANAEROBES BROTH WITH BLOOD

Technical Data #2458a / 98.06.25

BHI anaerobes broth with blood is used for cultivation and maintenance of anaerobic microorganisms. Yeast extract enhances growth of many bacteria and hemin-vitamin K1 improve anaerobes growth. The addition of L-cysteine reduces oxygen tension for anaerobes growth.

FORMULA
in grams per liter purified filtered water

Brain Heart Infusion/Meat Peptone	12,0
Casein Peptone	15,0
Yeast Extract	5,0
Dextrose	2,0
Sodium Chloride	5,5
Dipotassium Phosphate	2,5
Cystein HCl	0,5
Supplements
Hemin	0,005
Vitamin K	0,010
Defibrinated Sheep Blood	50 ml

pH 7,4 +/- 0,2 à 25º C

This approximate formula may be adjusted and/or enriched to obtain best results.

PRECAUTIONS
This medium is for IN VITRO diagnostic use only.

STORAGE
Store prepared media at 2-8º C protected from direct light. Medium must not be used if there are signs of contamination or if the expiry date has passed.

PROCEDURE
Bring tubes to room temperature before inoculation.

1. Inoculate specimens as soon as possible. Tighten the caps.
2. Incubate at 35º C for 24-72 hours and then up to 7 days
3. Examine tubes daily for visual signs of growth.

QUALITY CONTROL
Expected results after 48 hrs at 35ºC

Organisms	ATCC	Growth
Bacteroides fragilis	25285	+
Bacteroides levii	29147	+
Bacteroides vulgatus	8482	+
Clostridium novyi	7659	+
Clostridium perfringens	13124	+

LIMITATIONS OF METHOD
This is only a part of the identification. Other tests may be required.

REFERENCES

1. Balows,A., Hausler Jr. W.J., Herrmann, K.L., Isenberg, H.D., Shadomy, H.J., 1991. Manual of Clinical Microbiology 5th ed. American Society for Microbiology Washington, D.C.
2. Finegold S.M, Baron, E.J. 1986 Bailey and Scott's Diagnostic Microbiology 7th ed. C.V. Mosby.
3. Mc Faddin, J.F. 1985 Media for isolation-cultivation-identification-maintenance of medical bacteria. Vol. 1 Williams & Wilkins, Baltimore

CATALOGUE NUMBERS

Dehydrated media	500 g	QB-39-0305
Prepared media
Tubes 16x125 mm, 5 ml	10/pkg	2458
Supplements
Vitamin K-Hemin, 5ml	10/pkg	8752
Defibrinated Sheep Blood	50 ml	4245

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