COOKED MEAT DEXTROSE BROTH

Technical Data #2644a / 98.06.30

Cooked Meat Dextrose Broth is used for cultivation of anaerobic microorganisms using GLC (gas-liquid chromatography) to observe volatile fatty acid and alcohol production. Because of increased dextrose, this medium is not suitable for maintenance of anaerobes stock culture.

FORMULA
in grams per liter purified filtered water

Beef Heart	98,0
Meat Peptone	10,0
Casein Peptone	10,0
Dextrose	5,0
Sodium Chloride	5,0
Enrichments
Hemin	0,005
Vitamin K	0,0005

pH 7,2 +/- 0,2 à 25º C

This approximate formula may be adjusted and/or enriched to obtain best results.

PRECAUTIONS
This medium is for IN VITRO diagnostic use only.

STORAGE
Store prepared media at 2-8º C protected from direct light. Medium must not be used if there are signs of contamination or if the expiry date has passed. Store dehydrate powder in a dry place in tightly-sealed containers at 2-25º C. Do not use dehydrated media if expiry date has passed.

DIRECTIONS
Suspend 125 grams in 1 liter of purified water. Add 3 grams of dextrose and 10 ml of enrichment Vitamin K-Hemin (8751). Allow to stand for 15 minutes until all particles are wetted. Sterilize at 121º C for 15 minutes. Cool to 45-50º C. Mix thouroughly and dispense into sterile culture tubes.

PROCEDURE
Prior to inoculation, put the tubes, with loosened caps, in boiling water for 15 minutes and cooling to room temperature.

1. Inoculate specimens as soon as possible. Tighten the caps.
2. Incubate at 35º C for 24-72 hours
3. Examine tubes daily for visual signs of growth.

QUALITY CONTROL
Expected results after 48 hrs at 35ºC

Organisms	ATCC	Growth
Bacteroides fragilis	25285	+
Bacteroides levii	29147	+
Bacteroides vulgatus	8482	+
Clostridium novyi	7659	+
Clostridium perfringens	13124	+

LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.

REFERENCES

1. Balows,A., Hausler Jr. W.J., Herrmann, K.L., Isenberg, H.D., Shadomy, H.J., 1991. Manual of Clinical Microbiology 5th ed. American Society for Microbiology Washington, D.C.
2. Dowell, V.R., Lombard, G.L., Thompson, F.S., Armfield, A.Y. 1977 Media for isolation, characterization and identification of obligately anaerobic bacteria. CDC laboratory manual. Center for Disease Control, Atlanta
3. Holdeman, L.V., Cato, E.P., Moore, W.E.C. 1977 Anaerobe laboratory manual 4th ed. Anaerobe Laboratory. VPI, Blacksburg
4. Mc Faddin, J.F. 1985 Media for isolation-cultivation-identification-maintenance of medical bacteria. Vol. 1 Williams & Wilkins, Baltimore

CATALOGUE NUMBERS

Prepared media
Tubes, 16x125 mm, 10 ml	100/box	2644
Enrichment Vitamin K-Hemin, 5 ml	10/pkg	8751

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