BILE ESCULIN AZIDE BROTH

Technical Data #2886a / 2000.09.26

Bile Esculin Azide Broth is a highly selective culture medium used for the isolation of group D streptococci. Esculin hydrolysed by group D enterococci give a dark brown colour. Gram-positive bacteria other than enterococci and Gram-negative are inhibited by sodium azide.

FORMULA
in grams per liter purified filtered water

Casein Peptone	17,0
Meat Peptone	3,0
Yeast Extract	5,0
Oxgall	10,0
Esculin	1,0
Sodium Chloride	5,0
Sodium Citrate	1,0
Ferric Ammonium Citrate	0,5
Sodium Azide	0,25

pH 7,0 +/- 0,2 à 25º C

This approximate formula may be adjusted and/or enriched to obtain best results.

PRECAUTIONS
This medium is for IN VITRO diagnostic use only.

STORAGE
Store prepared media at 2-8º C protected from direct light and dehydrated powder, in a dry place in tightly-sealed containers at 2-25º C.

SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.

DIRECTIONS
Suspend 42,8 g in 1000 ml of purified water. Heat with frequent agitation and boil for one minute. Mix thouroughly and dispense into culture tube. Sterilize at 121º C for 15 minutes. Cool to 45-50º C.

PROCEDURE
Bring prepared medium at room temperature before inoculation.

1. Directly, inoculate the medium w/specimen.
2. Incubate at 35º C for 24 hours.

QUALITY CONTROL
Expected results after 24 hrs at 35ºC

Organisms	ATCC	Growth	Blackening
Streptococcus faecalis	29212	+	+
Streptococcus pyogenes	19615	- or partial	-
Escherichia coli	25922	- or partial	-

LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.

REFERENCES

1. Isenberg, H.D., D. Goldberg, J. Sampson 1970 Laboratory studies with a selective enterococcus medium. Appl. Microbiol. 20(3):433
2. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.: American society for Microbiology.
3. Mac Faddin, Jean F. 1985 Media for Isolation-Cultivation-dentification-Maintenance of Medical Bacteria. Vol. I, Baltimore, MD.: Williams & Wilkins.
4. N.C.C.L.S. 1990 Quality Assurance for Commercially Prepared Microbiological Culture Media Approved Standard. Vol. 10, No. 14, NCCLS Document M22-A.

CATALOGUE NUMBERS

Dehydrated media	500 g	QB-39-0119
Prepared media
Tubes 16X125 mm, 5 ml	10/pkg	2886

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