FASTIDIOUS ANAEROBE BROTH (F.A.B.)

Technical Data #2960a / 98.07.09

The F.A.B. Broth is a liquid medium used for cultivation of anaerobes microorganisms. This medium is very rich in nutrients from the selected peptones. L-Cystein and sodium thioglycollate reduce the Eh of the medium, agar decreases oxygen tension and resazurin is the redox indicator.

FORMULA
in grams per liter purified filtered water

Casein Peptone	10,0
Meat Peptone	5,0
Yeast Extract	10,0
Sodium Chloride	2,5
L-Cystein HCl	0,5
Sodium Bicarbonate	0,4
Sodium Thioglycollate	0,5
Resazurin	0,001
Agar, Purified	0,75

pH 7,2 +/- 0,2 à 25º C

This approximate formula may be adjusted and/or enriched to obtain best results.

PRECAUTIONS
This medium is for IN VITRO diagnostic use only.

STORAGE
Store prepared media at 2-8º C protected from direct light. Medium must not be used if there are signs of contamination or if the expiry date has passed. Store dehydrate powder in a dry place in tightly-sealed containers at 2-25º C. Do not use dehydrated media if it is caked and if expiry date has passed.

DIRECTIONS
Suspend 29,7 grams in 1000 ml of purified water. Heat gently with frequent agitation and boil for one minute. Mix gently and dispense into culture tubes. Sterilize at 121º C for 15 minutes.

PROCEDURE
Boil medium for 15 minutes and cool to room temperature before use.

1. Inoculate specimens as soon as possible. Tighten the caps.
2. Incubate at 35º C for 24-72 hours and then, up to 7 days.
3. Examine tubes daily for visual signs of growth.

QUALITY CONTROL
Expected results after 48 hrs at 35ºC

Organisms	ATCC	Growth
Bacteroides fragilis	25285	+
Bacteroides levii	29147	+
Bacteroides vulgatus	8482	+
Clostridium novyi	7659	+
Clostridium perfringens	13124	+
Peptostreptococcus anaerobius	27337	+

LIMITATIONS OF METHOD
Boil medium if more than 1/3 of the volume is oxidized. Do not heat tubes more than once to drive off absorbed oxygen. This is only a part of the identification. Other tests may be required.

REFERENCES

1. Ganguli, L.A., W. O'Hare, W.A. Hyde, 1984 Rapid detection of bacteraemia by early subculture. J. Med. Microbiol. 17:311-315
2. Ganguli, L.A., M.G.L. Keaney, W.A. Hyde, B.J. Fraser 1985 More rapid identification of bacteraemia by manual rather than radiometric methods. J. Clin. Pathol. 38:1146-1149.
3. Ganguli, L.A., L.J. Turton, G.S. Tillotson 1982 Evaluation of Fastidious Anaerobe Broth as a blood culture medium. J. Clin. pathol. 35:458-461.
4. Gould, J.H., B.I. Duerden 1983 Blood culture- current state and future prospects. J. Clin. Pathol. 36:963-977.
5. Junt, G.H., E.H. Price 1982 Comparaison of a home made blood culture broth containing a papain digest of liver with four commercially available media, for the isolation of anaerobes from simulated paediatoic blood cultures. J. Clin. Pathol. 35:1142-1149.
6. Tillotson, G.S. 1981 Evaluation of ten commercial blood culture systems to isolate a pyridoxal dependant streptococcus. J. Clin. Pathol. 34:930-934.

CATALOGUE NUMBERS

Dehydrated media	500 g	QB-39-1719
Prepared media
Tubes 16x125 mm, 10 ml	10/pkg	2960
FAB W/Cooked Meat Broth, Tubes 16x125mm, 10 ml	10/pkg	2994

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