B.H.I.A. CHLORAMPHENICOL 6% SHEEP BLOOD AGAR

Technical Data #2968a / 2000.11.01

BHIA Chloramphenicol 6% Sheep Blood Agar is a solid, selective medium used for yeasts and molds in cutaneous and respiratory specimens. Chloramphenicol inhibits normal flora growth.

FORMULA
in grams per liter purified filtered water

Brain Heart Infusion	10,0
Casein Meat Peptone	17,5
Sodium Chloride	5,0
Dextrose	2,0
Sodium Phosphate, dibasic	2,5
Agar	15,0
Defibrinated Sheep Blood	60,0 ml
Antibiotics
Chloramphenicol	0,05

pH 7,4 +/- 0,2 à 25º C

This approximate formula may be adjusted and/or enriched to obtain best results.

PRECAUTIONS
This medium is for IN VITRO diagnostic use only.

STORAGE
Store prepared media at 2-8° C protected from direct light and dehydrated powder, in a dry place, in tightly-sealed containers at 2-25° C.

SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.

DIRECTIONS
Suspend 52 g of dehydrated media into 940 ml of purified filtered water. Add chloramphenicol (QB-39-1116). Heat with frequent agitation and boil for one minute. Sterilize at 121° C for 15 minutes. Cool to 45-50° C and add 60 ml of Defibrinated Sheep Blood (#4147). Mix gently and dispense into sterile culture tubes.

PROCEDURE
Prior to inoculate, the prepared media should be brought to room temperature.

1. Inoculate tubes with specimens as soon as possible.
2. Incubate at 25° C for up to 4-6 weeks,with loosened caps.

QUALITY CONTROL
Results after 7 days at 25ºC

Organisms	ATCC	GROWTH
Candida albicans	10231	+
Trichophyton mentagrophytes	9533	+
Aspergillus niger	16404	+
Escherichia coli	25922	- or partial

LIMITATIONS OF METHOD
This medium is only a part of identification. Other tests may be required.

REFERENCES

1. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.: American Society for Microbiology.
2. Mac Faddin, Jean F., Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria Vol.1 1985 Baltimore, MD. Williams & Wilkins.
3. N.C.C.L.S. Quality Assurance for Commercially prepared Microbiological Culture Media N.C.C.L.S. Publication M22-A Approved Standard 1990 Vol.10 No.14.

CATALOGUE NUMBERS

Dehydrated media	500 grams	QB-39-0206
Chloramphenicol	500 grams	QB-39-1116
Defibrinated Sheep Blood	100 ml	4147
Prepared media
Tubes, 20x125 mm, 10 ml	10/pkg	2968

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