CARY BLAIR TRANSPORT MEDIUM

Technical Data #2981a / 2000.11.01

Cary Blair Transport Medium is a semi-solid medium used for maintenance of various pathogens and enteric pathogens bacteria as Campylobacter, Salmonella and Shigella in stools specimens.

FORMULA
in grams per liter purified filtered water

Sodium Thioglycollate

1,5

Sodium Chloride

5,0

Sodium Phosphate, dibasic

1,1

Agar

5,0

pH 8,0 +/- 0,2 à 25º C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for IN VITRO diagnostic use only.


STORAGE
Store prepared media at 2-8° C protected from direct light and dehydrated powder, in a dry place, in tightly-sealed containers at 2-25° C.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.


DIRECTIONS
Suspend 12,6 g of dehydrated media into 991 ml of purified filtered water. Heat with frequent agitation and boil for one minute. Add 9 ml of a 1% Calcium Chloride Solution. DO NOT AUTOCLAVE. Dispense into sterile culture tubes and steam for 15 minutes.


PROCEDURE
Prior to inoculate, the prepared media should be brought to room temperature.

  1. Dip swabs and break into culture media.
  2. Inoculate the culture media as soon as possible and streak to obtain isolated colonies.
  3. Incubate as required.


QUALITY CONTROL
Results after 24 hrs at 25ºC

Organisms

ATCC

GROWTH

Streptococcus pyogenes

19615

+

Haemophilus influenzae

10211

+

Bacteroides fragilis

25285

+

Escherichia coli

25922

+

Salmonella typhimurium

14028

+

Shigella flexneri

12022

+


LIMITATIONS OF METHOD
This medium is only a part of identification. Other tests may be required.


REFERENCES

  1. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.: American Society for Microbiology.
  2. Mac Faddin, Jean F., Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria Vol.1 1985 Baltimore, MD. Williams & Wilkins.
  3. Finegold, S.M., and E.J. Baron. 1986 Bailey's and Scott's Diagnostic Microbiology. 7th ed. St. Louis. The C.V. Mosby Company.


CATALOGUE NUMBERS

Dehydrated media 500 grams QB-39-0708
     
Prepared media    
Tubes, 16x125 mm, 14 ml 10/pkg 2981
Tubes, 17x90 mm, 10 ml 10/pkg 2985

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